Ukurikije ihame ryo kugena azote ya Kjeldahl, hakenewe intambwe eshatu kugirango umuntu yiyemeze, aribyo igogora, kurigata no gutanga titre.
Gusya: Shyushya azote irimo ibinyabuzima (proteyine) hamwe na acide sulfurike yibanze hamwe na catalizator (sulfate y'umuringa cyangwa ibinini bya Kjeldahl igogora) kugirango ubore proteine. Carbone na hydrogène bihindurwamo umwuka wa dioxyde de carbone n'amazi kugirango bahunge, naho Nitrogen kama ihinduka ammonia (NH3) igahuzwa na aside sulfurike ikora sulfate ya amonium. (Amonium NH4 +)
Uburyo bwo gusya: gushyushya hamwe nubushyuhe buke bwo guteka, ibintu biri muri flask birimo karubone kandi birabura, kandi havamo ifuro ryinshi. Ifuro imaze kubura, ongera imbaraga zumuriro kugirango ugumane gato. Iyo amazi ahindutse ubururu-icyatsi kandi gisobanutse, komeza ushushe kuri 05-1h, hanyuma ukonje nyuma yimpera. (Urashobora gukoresha igikoresho cyikora cyikora kugirango urangize imirimo yo gutunganya mbere)
Gusibanganya: Igisubizo cyabonetse kivangwa nubunini buhoraho hanyuma kongerwaho na NaOH kurekura NH3 kubitandukanya. Nyuma ya kondegene, ikusanyirizwa mumuti wa boric.
Uburyo bwo gusibanganya: Ubwa mbere, icyitegererezo cyashizwemo kiravangwa, NaOH yongerwamo, na gaze ya amoniya ikorwa nyuma yo gushyushya yinjira muri kondenseri, hanyuma ikinjira mu icupa ryakira ririmo umuti wa aside ya boric nyuma yo guhunika. Ifite ammonium borate. .
Titration: Titrate hamwe na hydrochloric acide isanzwe yumuti uzwi, ubare ibirimo azote ukurikije ingano ya aside ya hydrochlorike yakoreshejwe, hanyuma uyigwize nibintu bihinduka kugirango ubone proteine. . hanyuma ukareba neza ikoreshwa ryibisubizo bisanzwe Umubare, kubara no gusesengura ibisubizo.)
Uburyo bwa titre: Tera igisubizo gisanzwe cya acide hydrochloric mumuti wa ammonium borate kugirango uhindure ibara ryumuti kuva mubururu-icyatsi kibisi gitukura.
DRK-K616 isesengura Kjeldahl isesengura nitorojeni ni isesengura ryubwenge bwikora kugirango igenure ibirimo azote ishingiye kuburyo bwa Kjeldahl. Irashobora gukoreshwa cyane mugutunganya ibiribwa, kubyara ibiryo, itabi, ubworozi, ifumbire yubutaka, gukurikirana ibidukikije, ubuvuzi, ubuhinzi, ubushakashatsi bwa siyansi, kwigisha, kugenzura ubuziranenge nizindi nzego hagamijwe gusesengura azote na proteyine muri macro na kimwe cya kabiri cya micro ingero. Irashobora kandi gukoreshwa kumunyu wa amonium, Kumenya acide fatty acide / alkali, nibindi. Iyo ukoresheje uburyo bwa Kjeldahl kugirango umenye icyitegererezo, bigomba kunyura muburyo butatu bwo gusya, kubitandukanya, no kubitanga. Kurandura no gutanga titre ninzira nyamukuru yo gupima DRK-K616 Kjeldahl isesengura rya azote. Ubwoko bwa DRK-K616 isesengura rya azote ya Kjeldahl ni uburyo bwo gupima no gukoresha nitorojeni ya sisitemu yo gupima nitorojeni yateguwe hakurikijwe uburyo bwa azote bwa Kjeldahl; iki gikoresho gitanga ubworoherane kubapimisha laboratoire mugikorwa cyo kumenya azote-proteyine. , Kandi ifite ibiranga imikoreshereze yizewe kandi yizewe; imikorere yoroshye no guta igihe. Imigaragarire yubushinwa ituma uyikoresha yoroha gukora, intera ninshuti, kandi amakuru yerekanwe arakungahaye, kugirango uyakoresha ashobore kumva vuba ikoreshwa ryigikoresho.
Igihe cyo kohereza: Gashyantare-25-2022
