CFX96TOUCH Fluorescent Quantitative PCR igikoresho

CFX96Touch fluorescent quantitative PCR irashobora gukoreshwa mubice bitandukanye byubushakashatsi bwo kugereranya aside nucleic, isesengura ryerekana imiterere ya gene, kumenya ihinduka rya gene, kumenya GMO, no gusesengura ibicuruzwa byihariye.

Ibidukikije bikora:
1.1 Ubushyuhe bwo gukora: 5-31 ° C.
1.2 Gukora nubushuhe: ubushuhe bugereranije ≤ 80%
1.3 Imbaraga zakazi: 100-240 VAC, 50-60Hz
Imikorere nibisabwa bya tekinike ya CFX96Koraho fluorescence yuzuye PCR
3.1 Imikorere nyamukuru (* nicyo kimenyetso kigomba kubahirizwa)
* 3.1.1 Imiyoboro itandatu yikizamini, 5% PCR irashobora kugerwaho, kandi genes 5 zigenewe zishobora kumenyekana icyarimwe, kandi umuyoboro udasanzwe wa FRET wamenyekanye icyarimwe.
* 3.1.2 Hamwe nubushyuhe bwa dinamike gradient imikorere ya PCR, urashobora gukoresha ubushyuhe 8 butandukanye icyarimwe, buri bushyuhe bwubushyuhe.
3.1.3 Gufungura reagent zuzuye, ubushakashatsi butandukanye nubuvuzi bwa clinique birakoreshwa;
3.1.4 Birakwiriye kuburyo butandukanye bwa fluorescence nka Taqman, Beacon ya Molecular, Fret probe, Sybr Green i, nibindi .;
3.1.5 Fungura, 0.2ml umuyoboro umwe, octal, isahani 96-nziza, nibindi
* 3.1.6 irashobora gukora yigenga, mubyukuri ikora kumurongo, ntagikeneye guhuza mudasobwa kugirango ikurikirane PCR fluorescent amplification curve mugihe nyacyo;
3.2 Ibisabwa byingenzi bya tekiniki (* nicyo kimenyetso kigomba kuba cyujuje)
* 3.2.1 Ubushobozi bw'icyitegererezo: 96 × 0.2ml, ibisobanuro bisanzwe 96 amasahani meza (12 × 8) arashobora gukoreshwa;
3.2.2 Ibikoresho Ubwoko: 0.2ml umuyoboro umwe, umunani uhuza, amasahani 96-meza, nibindi.
3.2.3 Sisitemu yo Kwitwara: 1-50μL (bisabwa 10-25 μL);
* 3.2.4 Inkomoko yumucyo: LED esheshatu zifite akayunguruzo;
* 3.2.5 Detector: Diode esheshatu zifotora hamwe na filteri;
* 3,2,6 ya litiro yo gukonjesha: 5 ° C / amasegonda;
3.2.7 Igipimo cyo kugenzura ubushyuhe: 0 -100 ° C;
3.2.8 Ubushyuhe Bwuzuye: ± 0.2 ° C (90 ˚C);
3.2.9 Uburinganire bwubushyuhe: ± 0.4 ° C (90 ˚C mumasegonda 10);
* 3.2.10 Igikorwa cya Dynamic Temperature Gradient Imikorere: Koresha ubushyuhe 8 butandukanye icyarimwe; igipimo cyo kugenzura ubushyuhe bwa gradient: 30 -100 ° C; itandukaniro ry'ubushyuhe butandukanye: 1 - 24 ° C; ubushyuhe bwa gradient incubation igihe: kimwe;
3.2.11 kwishima / gusohora intera yumurambararo: 450-730 nm;
3.2.12 Ibyiyumvo: Kopi imwe ya gen muri genome yabantu irashobora kumenyekana;
3.2.13 Urwego rudasanzwe: ingano 10;
3.2.14 Erekana: 8.5 inch ya ecran ikoraho;
3.2.15. ;
3.2.16 Kohereza amakuru hanze: Excel, Ijambo, cyangwa PowerPoint. Raporo y'abakoresha ikubiyemo igenamiterere, ibishushanyo, n'ibisubizo by'amakuru y'ibisubizo, bishobora gucapurwa cyangwa kubikwa nka PDF;
* 3.2 Byerekana rwose uburebure bufitanye isano ya chromatin imiterere na gene imvugo;