5425R Umuvuduko mwinshi wa Centrifuge

Amen's 5425R yihuta cyane ya centrifuge (Centrifuge5425R), ubwoko bwa buto, ubwoko bwakonje, rotor FA-24X2, 230V / 50-60Hz (CN), interineti ikora buto, gukora isuku byoroshye. Gukonja.

Amen's 5425R yihuta cyane ya centrifuge (Centrifuge5425R), ubwoko bwa buto, ubwoko bwakonje, rotor FA-24X2, 230V / 50-60Hz (CN), interineti ikora buto, gukora isuku byoroshye. Gukonja. Nkibicuruzwa byakurikiyeho bizwi cyane bya Centrifuge 5424 / 5424R, ubu buryo bubiri bushya burimo ibicuruzwa byose ukunda moderi zabanjirije iyi, hamwe na rotor nshya hamwe nibiranga software, bigatuma akazi kawe ka buri munsi karushaho gukora neza kandi kunezeza. Igishushanyo cyihariye cya Centrifuge 544 ikonjesha ikirere itanga imikorere ituje cyane kugirango itange akazi keza. Mugihe cyiminota 12, centrifugation irashobora kugerwaho, kandi igishushanyo mbonera cyamajwi kirakoreshwa, kandi urusaku rusanzwe ni 45dB (a) gusa. Ibikorwa byigihe gito birahagije kugirango urangize intambwe ya centrifugation yibikoresho bisanzwe bya ADN / RNA. Iyo wiruka umwanya muremure, umuyaga ukonjesha ukwirakwizwa neza mugihe gisigaye.

Sisitemu nziza yo gukonjesha Centrifuge 5425R yemeza neza ko itandukanyirizo ryiza, ndetse no mumuvuduko ntarengwa wa rotor, irashobora gukonjeshwa neza kuri 4 ° C. Gusa igihe gito cyiminota 8 mbere yo gukonjesha (~ 21 ° C kugeza 4 ° C), centrifuge irashobora gukoreshwa igihe icyo ari cyo cyose. Rotor zitandukanye ubu zirimo ibintu byinshi byashyizwe mubikorwa, harimo ibinyabuzima byifashishwa mu binyabuzima, nka ADN / RNA cyangwa kweza poroteyine, imvura igwa ya bagiteri, ikomoka kuri ml 5 ya tebes yipimisha, na selile, hamwe nubushakashatsi bwakozwe, urugero, PCR pre -ubuvuzi / nyuma yo kuvurwa hamwe na sisitemu ya QPCR yubaka mumazi ya 0.2 ml, isahani 8-iriba, cyangwa isahani ya 96-iriba. Eppendorfkitrotor® (FA-18X2-Kit) ikoreshwa kuri 18 x centrifugal inkingi na 1.5 / 2.0ml ya centrifuge (ntarengwa 18,565 xg). Iyi rotor idasanzwe ikemura ibibazo bisanzwe bihura nabyo mugihe ukoresheje Miniprep izunguruka inkingi: Igifuniko cya kontineri ifunguye yaciwe murwego rwo gutora. Mubisanzwe ibiba iki kibazo nuko rotor gakondo idashobora gutanga inkunga ihagije kumutwe wa centrifuge yafunguwe mugihe cya centrifugation. Eppendorfkitrotor ifite impande yazamuye ishyirwa hejuru mugihe cya centrifugation, ntabwo rero izacibwa. Guhera ubu, igifuniko cya kontineri yawe kizaguma kurubuga rwacyo. Rotor FA-10 × 5 ikoreshwa mumiyoboro ya 10 × 5.0mleppendorf centrifuge (kugeza kuri 21.300 xg), kandi bagiteri, umusemburo wumuco cyangwa selile bisarurwa muri Eppendorftubes® 5.0ml, kandi ADN / RNA hamwe na ADN ya plazimasi ikoreshwa kugirango bibe biroroshye cyane. Adapters nayo itangwa kugirango igere kuri centrifugation ukoresheje 1.5 / 2 mL ya centrifuge hamwe na HPLC na CRYO centrifugal itabanje gusimbuza rotor.

5425R yihuta cyane ya centrifuge iranga:
1, umuyaga mwinshi EppendorfquickLock® igifuniko, gukora ingero ziroroshye cyane, umutekano;
2, ibintu byiza bya software biranga (nko kwihuta kwihuta no guhagarara kuri feri, buto 3 ya progaramu, nta shortcuts ya buto ikomeza);
3, kuzamura porogaramu ya rotor (urugero, 5.0ml rotor, ibereye urwego rwumuyoboro wa PCR);
4, Igishushanyo cya OptiBowl®, menya neza ko imikorere ya mute, nubwo igifuniko cya rotor nacyo cyatuje cyane, kanda witonze kugirango ufunge igifuniko cya centrifugal, nta mbaraga zikomeye cyane;
5, ubushobozi bwa rotor ntarengwa: 10 × 5.0ml umuyoboro wa centrifuge
6, imbaraga ntarengwa zijyanye na centrifugal: 21,300 × g (15,060 rpm)
7,5425R Gukonjesha Umuvuduko mwinshi Centrifuge imikorere idasanzwe, sisitemu nziza yo gukonjesha yo gutunganya 0.2-5 mL yubushyuhe bwubushyuhe, tekinoroji ya compressor yemewe, igabanya kunyeganyega, kurinda ingero. Ikirere cy'ubushyuhe: -10 c kugeza + 40 ° C.

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